quantitative sandwich commercial elisa kits  (Cusabio)

Journal: Frontiers in Public Health

Article Title: Serum Angiopoietin-Like Protein 4: A Potential Prognostic Biomarker for Prediction of Vascular Invasion and Lymph Node Metastasis in Cholangiocarcinoma Patients

doi: 10.3389/fpubh.2022.836985

Figure Lengend Snippet: (A) Venn diagram showing 123 proteins express in both, 41 and 112 proteins uniquely express in Group A patients (CCA with ≤ 1 year survival time) and Group B patients (CCA with >1 year survival time). (B) Flow diagram of selection of candidate secreted proteins in Group A and Group B patients. Proteins identify in serum/plasma database and having signal peptide or non-classical proteins as candidate secreted proteins. ANGPTL4 is selected from Group B patients which express highest signal intensity of LC-MS/MS among candidate secreted proteins.

Article Snippet: To correlate serum ANGPTL4 level between dot blot assay and ELISA, we determined ANGPTL4 level in randomly selected 20 samples using ANGPTL4 commercially quantitative sandwich ELISA kit (DY3485, R and D systems, USA) according to manufacturer's instructions.

Techniques: Selection, Clinical Proteomics, Liquid Chromatography with Mass Spectroscopy

Journal: Frontiers in Public Health

Article Title: Serum Angiopoietin-Like Protein 4: A Potential Prognostic Biomarker for Prediction of Vascular Invasion and Lymph Node Metastasis in Cholangiocarcinoma Patients

doi: 10.3389/fpubh.2022.836985

Figure Lengend Snippet: (A) Representative immunohistochemical staining of ANGPTL4 in CCA tissues (magnification, x400). Hepatocytes (H) strongly express ANGPTL4 while normal bile duct (NBD) express weak positive staining and CCA cells express strongly positive staining. (B) H-scores of ANGPTL4 in normal and CCA tissues using immunohistochemistry. Mann-Whitney U -test was used to compare H-scores between normal and cancerous tissues of ANGPTL4. Statistical significance ( p < 0.05).

Article Snippet: To correlate serum ANGPTL4 level between dot blot assay and ELISA, we determined ANGPTL4 level in randomly selected 20 samples using ANGPTL4 commercially quantitative sandwich ELISA kit (DY3485, R and D systems, USA) according to manufacturer's instructions.

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry, MANN-WHITNEY

Journal: Frontiers in Public Health

Article Title: Serum Angiopoietin-Like Protein 4: A Potential Prognostic Biomarker for Prediction of Vascular Invasion and Lymph Node Metastasis in Cholangiocarcinoma Patients

doi: 10.3389/fpubh.2022.836985

Figure Lengend Snippet: Association between ANGPTL4 expression and clinicopathological data of CCA patients.

Article Snippet: To correlate serum ANGPTL4 level between dot blot assay and ELISA, we determined ANGPTL4 level in randomly selected 20 samples using ANGPTL4 commercially quantitative sandwich ELISA kit (DY3485, R and D systems, USA) according to manufacturer's instructions.

Techniques: Expressing

Journal: Frontiers in Public Health

Article Title: Serum Angiopoietin-Like Protein 4: A Potential Prognostic Biomarker for Prediction of Vascular Invasion and Lymph Node Metastasis in Cholangiocarcinoma Patients

doi: 10.3389/fpubh.2022.836985

Figure Lengend Snippet: (A) Serum ANGPTL4 level of HC and CCA patients by dot blot assay. The median ± quartile deviation, 0.1634 ± 0.1739 AU (0.0193–0.9424 AU), in healthy control and 0.6017 ± 0.3767 AU (0.0984–2.9526 AU) in CCA patients' group. To normalize the expression level, the ratio of the intensity of each dot blot to that of the positive control (pooled CCA serum) was calculated and determined as the arbitrary unit (AU). The difference of serum ANGPTL4 level between HC and CCA by Mann-Whitney U-test. (B) ROC curve of serum ANGPTL4 level as a potential biomarker for prediction of CCA in comparison group of HC, sensitivity = 80.0% and specificity = 72.7% (AUC = 0.825, 95% CI 0.751–0.899, p < 0.0001). Statistical significance ( p < 0.05).

Article Snippet: To correlate serum ANGPTL4 level between dot blot assay and ELISA, we determined ANGPTL4 level in randomly selected 20 samples using ANGPTL4 commercially quantitative sandwich ELISA kit (DY3485, R and D systems, USA) according to manufacturer's instructions.

Techniques: Dot Blot, Control, Expressing, Positive Control, MANN-WHITNEY, Biomarker Discovery, Comparison

Journal: Frontiers in Public Health

Article Title: Serum Angiopoietin-Like Protein 4: A Potential Prognostic Biomarker for Prediction of Vascular Invasion and Lymph Node Metastasis in Cholangiocarcinoma Patients

doi: 10.3389/fpubh.2022.836985

Figure Lengend Snippet: Association between serum ANGPTL4 level and clinicopathological parameters in CCA patients.

Article Snippet: To correlate serum ANGPTL4 level between dot blot assay and ELISA, we determined ANGPTL4 level in randomly selected 20 samples using ANGPTL4 commercially quantitative sandwich ELISA kit (DY3485, R and D systems, USA) according to manufacturer's instructions.

Techniques:

Journal: Frontiers in Public Health

Article Title: Serum Angiopoietin-Like Protein 4: A Potential Prognostic Biomarker for Prediction of Vascular Invasion and Lymph Node Metastasis in Cholangiocarcinoma Patients

doi: 10.3389/fpubh.2022.836985

Figure Lengend Snippet: ROC curve analysis of biomarkers (ANGPTL4, CA 19-9, and CEA) for prediction of CCA in comparison group of (A) No vascular invasion vs. Vascular invasion, (B) No lymph node metastasis vs. Lymph node metastasis. Bold values * Statistical significance ( p < 0.05).

Article Snippet: To correlate serum ANGPTL4 level between dot blot assay and ELISA, we determined ANGPTL4 level in randomly selected 20 samples using ANGPTL4 commercially quantitative sandwich ELISA kit (DY3485, R and D systems, USA) according to manufacturer's instructions.

Techniques: Comparison

Journal: Frontiers in Public Health

Article Title: Serum Angiopoietin-Like Protein 4: A Potential Prognostic Biomarker for Prediction of Vascular Invasion and Lymph Node Metastasis in Cholangiocarcinoma Patients

doi: 10.3389/fpubh.2022.836985

Figure Lengend Snippet: Predictive values of serum ANGPTL4, CA 19-9, and CEA levels for vascular invasion and lymph node metastasis of CCA patients, based on the optimal cutoff derived from ROC analysis and YI calculation.

Article Snippet: To correlate serum ANGPTL4 level between dot blot assay and ELISA, we determined ANGPTL4 level in randomly selected 20 samples using ANGPTL4 commercially quantitative sandwich ELISA kit (DY3485, R and D systems, USA) according to manufacturer's instructions.

Techniques: Derivative Assay

Journal: Frontiers in Public Health

Article Title: Serum Angiopoietin-Like Protein 4: A Potential Prognostic Biomarker for Prediction of Vascular Invasion and Lymph Node Metastasis in Cholangiocarcinoma Patients

doi: 10.3389/fpubh.2022.836985

Figure Lengend Snippet: Univariate and multivariate regression analysis of clinical parameters associated with vascular invasion.

Article Snippet: To correlate serum ANGPTL4 level between dot blot assay and ELISA, we determined ANGPTL4 level in randomly selected 20 samples using ANGPTL4 commercially quantitative sandwich ELISA kit (DY3485, R and D systems, USA) according to manufacturer's instructions.

Techniques:

Journal: Frontiers in Public Health

Article Title: Serum Angiopoietin-Like Protein 4: A Potential Prognostic Biomarker for Prediction of Vascular Invasion and Lymph Node Metastasis in Cholangiocarcinoma Patients

doi: 10.3389/fpubh.2022.836985

Figure Lengend Snippet: Univariate and multivariate regression analysis of clinical parameters associated with lymph node metastasis.

Article Snippet: To correlate serum ANGPTL4 level between dot blot assay and ELISA, we determined ANGPTL4 level in randomly selected 20 samples using ANGPTL4 commercially quantitative sandwich ELISA kit (DY3485, R and D systems, USA) according to manufacturer's instructions.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: NLRC5 Serves as a Pro-viral Factor During Influenza Virus Infection in Chicken Macrophages

doi: 10.3389/fcimb.2020.00230

Figure Lengend Snippet: NLRC5 protein is increased in LPAIV-infected chicken macrophages. Chicken macrophage MQ-NCSU cells were infected with the H7N2 or H5N2 AIVs for 24 h. (A) NLRC5 protein was quantified using ELISA. (B) Fluorescence photomicrographs of (B) mock-infected cells stained for NLRC5 and (C) LPAIV H5N2-infected macrophage cells. The blue color represents DAPI-nuclei specific staining and the green color represents FITC-NLRC5 specific staining. Data points are mean of three biological replicates with error bars showing standard deviation. *** p ≤ 0.001, **** p ≤ 0.0001.

Article Snippet: In addition, to quantify the expression of NLRC5, a quantitative sandwich ELISA was performed using a commercial ELISA kit (MyBioSource, San Diego, CA, USA) as per the manufacturer's instructions.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Fluorescence, Staining, Standard Deviation

Journal: Neuropsychiatric Disease and Treatment

Article Title: Simvastatin pretreatment ameliorates t-PA–induced hemorrhage transformation and MMP-9/TIMP-1 imbalance in thromboembolic cerebral ischemic rats

doi: 10.2147/NDT.S199371

Figure Lengend Snippet: Simvastatin ameliorates t-PA-induced blood MMP-9/TIMP-1 ratio imbalance. Rats were pretreated with SIM (60 mg/kg/d) for the SIM+ group or water for the SIM- group for two weeks, then rats were received t-PA (10 mg/kg) for the t-PA+ group or normal saline for the t-PA- group 3 hrs after operation. Blood samples were collected via abdominal aorta puncture before sacrificed. ( A ) ELISA analysis of MMP-9 and TIMP-1 protein expression. ( B ) Real-time PCR analysis of MMP-9 and TIMP-1 mRNA levels. ( C ) The blood MMP-9/TIMP-1 protein ratio. ( D ) The blood MMP-9/TIMP-1 mRNA ratio. Bars express the mean ± SD of duplicates (n=6). ** p <0.01 t-PA alone versus t-PA plus SIM; && p <0.01 t-PA alone versus t-PA-/SIM-; ## p <0.01 versus sham group. Abbreviation: SIM, simvastatin.

Article Snippet: The plasma levels of secreted MMP-9 and TIMP-1 protein were determined using commercially available quantitative sandwich ELISA kits obtained from R&D Systems, USA.

Techniques: Saline, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction